Offer Ends In: 45 Days 06: 20: 11. We would like to show you a description here but the site won’t allow us. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Microplate Readers. Absorbance (monochromator). (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. 25 to 3. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Last lab had ours for years and never had a problem with it. It can measure absorbance from 230 -1000 nm. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 0) OD (0. Fixes: General:SpectraMax M2 and M2e Multi-Mode. Fluorescent Polarization: Solid black plates, as above. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. I am wondering if the type of 96-well plate matters. We suggest Corning 384-well plates Cat. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. S. Tecan Infinite F200 Fluorescence Microplate Reader. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Microplate Reader, For Lab. SpectraMax M5), place the adapter in the plate carriage. 5 pM typical (0. 1 cm) Weight 27 lb. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Other technologies such as. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Model Description. Plate Formats: 6, 12, 24, 48, 96, 384 wells: Light Source: Xenon Flash Lamp (1 joule/flash)The SpectraMax® iD3 Multi Mode Microplate . Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. We would like to show you a description here but the site won’t allow us. 5. The kit includes everything you need: reagents, buffers and a microplate. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. Python 3. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. 9000-0756. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. The purple holder is used for reading non-transparent plates from the top (e. Add to Cart. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. The SpectraMax® M2 and M2e Multimode. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 7°C (gradient heating design). variety of. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The Cut-off filter for this pair is showing 530nm in this machine. SpectraMax Microplate Readers. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. FAST AND ACCURATE METERTECH Microplate Reader $3,650. FI assay. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 209, but in 96-well plate was 0. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 1. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 04 to 3. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. A). These measurements can include parameters such as absorbance, fluorescence intensity and. 0077 OD 0. Equipment Details. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. On microplate readers,. Plate reader settings for NanoOrange assay. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. In addition, the SpectraMax iD5The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 0) OD (0. 00. Absorbance (monochromator) Fluorescence (monochromator; FI). The fluorescence intensity of MitoTracker® Red CM-H2XRos was measured with a fluorescence plate reader at excitation and. An assay plate containing a 15-point standard curve was read on the SpectraMax. 7. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Open and turn on the laptop. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. We therefore gathered salient. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. 0 out of 5 $ 9,995 $ 9,495. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Add to Cart. , a positive control). BioTek Synergy 2. UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. With optimized reagents, option to operate the readers. $ 2,499. SpectraMax Quant dsDNA Assay Kits. Note: In this user guide, SpectraMax ® refers to several SpectraMax . patent nos. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. Auto-calibration and self-diagnostics. Selecting your dsDNA. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Enables accurate fluorescence data with every run. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. The pre-configured protocols can be selected for each assay and. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Features. To schedule a training session please contact Ewa Folta-Stogniew. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Filters: Excitation and emission slides for 4 filters each. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Microplate Capacity. 0 × 107) RLU 0. 49 µM,. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 100 readings per well gives optimal Z’ values for this assay. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. The modular platform allows upgrading to expand functionality. plate over and over again. STAKMAX. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 020 OD 2. The drawing shows a standard microplate of 15 mm height covered with a lid. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The chamber contains. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Download PDF. 2. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. 1. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. Linear, Orbital Slow, and Orbital Fast. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Add to Cart. Appendix. 5,112,134 5,766,875. Conclusions. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Using a black. Bottom Monochromator: Fluorescein 2. . The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. 020 OD 2. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. Add to Cart. Double-click the unknown device with the yellow warning icon. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Brand: MOLECULAR DEVICES. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. The plate is set up for reading and images are acquired according to specified parameters. Add to Cart. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. Usage/Application: Lab. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. Get unrivalled sensitivity and reproducibility and elevate your microplate assay protocol with the Eppendorf Assay/Reader Plates. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Spectramax Multi-Mode Microplate Reader Performance Specifications. 315 can't find zero order. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Molecular Devices SpectraMax M2 ; Molecular Devices. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Offer. 2018. 25 to 3. D 4/21/06 9:53:29 AM . Endpoint, kinetic, spectrum. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. 67. Figure 1. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. E Models. 303 unable to cal dark current. Select the Driver tab and click Update Driver. SpectraMax ® M2 Multi-Mode Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. When a feature or capability applies to only certain readers, this exception is noted. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. was 1. Biotek FLx800 Fluorescence Microplate Reader. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. 58% for the detection module and 2. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. Three kits covering a wide range of dsDNA concentrations. The SpectraMax Plus 384 Microplate Reader for. A. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. Type Clear: Add to Cart. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Fluorescence read mode and Endpoint read type are already selected in the pre-configured protocol. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Because. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Model: SpectraMax M2. The SpectraMax 190 reader can be integrated with Molecular . Transcreener ® ADP. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. 6 % of reading 4. D 59 A. Category 2 and 3 microplate readers use gradient heating as well. 2. This can increase throughput and efficiency, thus saving time and space in the lab. ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. L evices evices orporation. 0 software was used to handle data analysis and control each instrument. standard spectral absorbance, fluorescence, and luminescence detection. 313 reference gain check fail. Table 1. Downloads. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Standard. Appendix A. 00. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax M2 and use thereof is covered by issued (U. When a. Other technologies such as imaging. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. For a higher degree of automation, the Automation. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Luminex Magpix. Published Dec 16, 2020 | Updated Dec 16, 2020. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2/m2e user guide SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM SpectraMax M2 & SpectraMax…plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). Read the microplate Step 1: Place the microplate in the reader. Molecular Devices i3x. 67. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Multimode microplate readers offer flexibility, and ease of use. The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as. Sale. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Skip to main content. Wide range of applications. Tecan’s. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Instructions. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Basic "FL Plate Reader start up protocol" (print and bring along for the. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. 0) OD (500 to 1100) RFU (5. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. Microplate Readers Include the M3, M4, M5, and M5. 1. 1. Package performance tests. 0 × 107) RLU 0. Fully tested with validations and warranty. BMG Labtech - THERMOstar. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 0 out of 5 $ 7,999. The entry should be SpectraMax GEMINI EM. Wavelength optimization. Microplate Reader Shelf. free tracer decreases, resulting in an overall increase in RFU values. 8: theMicroplate Reader & Luminometer Selection Guide. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Add to Cart. begin reading microplates with seven mouse clicks. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Prior to fluorescence analysis, the lid from the OmniTray plate was removed. 119. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. fluorescence). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. 3) At the bottom of the strip, click on "Show. Figure 1. U. Other features and benefits include: Eliminate changing out filters. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. The password is. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Refurbished. Microplate Formats: 6, 12, 24, 48, 96, 384. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE Summary of Contents for Molecular Devices SpectraMax M2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 5%in96-and384-wellmicroplates. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. A microplate reader asPython 3. Temperature Control: ambient from +4 to 45C. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader.